Partitioning the structural features that underlie expansin-like and elicitor activities of cerato-platanin

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A single amino acid mutation affects elicitor and expansins-like activities of cerato-platanin, a non-catalytic fungal protein

Cerato-platanin (CP) is a non-catalytic, cysteine-rich protein, the first member of the cerato-platanin family. It is a single-domain protein with a double Ψ/β barrel domain resembling the D1 domain of plant and bacterial expansins. Similarly to expansins, CP shows a cell wall-loosening activity on cellulose and can be defined as an expanisin-like protein, in spite of the missing D2 domain, nor...

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Phylogeny and molecular dating of the cerato-platanin-encoding genes

The cerato-platanin family consists of proteins that can induce immune responses, cause necrosis, change chemotaxis and locomotion and may be related to the growth and development of various fungi. In this work, we analyzed the phylogenetic relationships among genes encoding members of the cerato-platanin family and computed the divergence times of the genes and corresponding fungi. The results...

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Cerato-platanin family proteins: one function for multiple biological roles?

INTRODUCTION Despite the increasing number of sequenced genomes, cerato-platanin family proteins (CPPs) seem to exist only in fungi. Bacteria, oomycota, plants, and animals do not in fact possess CP homologs, whereas genes codifying for CPPs have been found, so far, in more than 50 fungal genomes (Chen et al., 2013). Among these fungi there are plant pathogens, such as Botrytis cinerea and Magn...

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The structure of the elicitor Cerato-platanin (CP), the first member of the CP fungal protein family, reveals a double ψβ-barrel fold and carbohydrate binding.

Cerato-platanin (CP) is a secretion protein produced by the fungal pathogen Ceratocystis platani, the causal agent of the plane canker disease and the first member of the CP family. CP is considered a pathogen-associated molecular pattern because it induces various defense responses in the host, including production of phytoalexins and cell death. Although much is known about the properties of ...

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The fungal cerato-platanin protein EPL1 forms highly ordered layers at hydrophobic/hydrophilic interfaces.

Cerato-platanin proteins (CPPs) and hydrophobins are two classes of small, secreted proteins that are exclusively found in fungi. CPPs are known as chitin-binding proteins, and were recently also shown to form protein layers at air/water interfaces, but the features of these layers were not investigated on the molecular level yet. In this study, by means of atomic force microscopy (AFM), EPL1, ...

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ژورنال

عنوان ژورنال: International Journal of Biological Macromolecules

سال: 2020

ISSN: 0141-8130

DOI: 10.1016/j.ijbiomac.2020.10.122